Sequence-Dependent Pausing of Single Lambda Exonuclease Molecules
نویسندگان
چکیده
منابع مشابه
Sequence-dependent pausing of single lambda exonuclease molecules.
Lambda exonuclease processively degrades one strand of duplex DNA, moving 5'-to-3' in an ATP-independent fashion. When examined at the single-molecule level, the speeds of digestion were nearly constant at 4 nanometers per second (12 nucleotides per second), interspersed with pauses of variable duration. Long pauses, occurring at stereotypical locations, were strand-specific and sequence-depend...
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ژورنال
عنوان ژورنال: Science
سال: 2003
ISSN: 0036-8075,1095-9203
DOI: 10.1126/science.1088047